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20 min later, the mice were anesthetized (24 % isoflurane) and placed in a dorsal position during imaging
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This timing may maximize the compounds effectiveness, as its working in concert with elevated growth hormone levels and the parasympathetic nervous system activation that occurs during sleep

The endogenous peptide concentration was estimated by taking the ratio of endogenous to single SIL-synthetic internal standard (hipMHC peptide) and multiplying by the concentration of hipMHC

During the Trump administration that followed, that funding was maintained, although it changed names just this last year or so, the brain initiative was cut
