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0.16 g/ml, 0.16 ng/ml) and its effect was assessed at the corresponding intervals

Int J Mol Sci (2022) 23(11)

Isolation and culture of T cells Peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors by gradient density centrifugation and then prepared at 3 10 6 cells/mL in T cell medium (X-VIVO TM 15, 10% fetal bovine serum, glutamax, human IL-2, human IL-7, and human IL-15)

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