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Furthermore, the synergistic action of disulfiram and Cu 2+ enhanced ROS production and activated the p38 mitogen-activated protein kinase (MAPK) signaling pathway
GAPDH was used for normalization, and experiments were repeated a minimum of three times
While a 1mg/ml concentration is a great starting point, the correct ratio is ultimately the one that makes the most sense for your specific experiments
After 24 hours of incubation, 4 displayed a cellular IC 50 value of 1.6 and 6.3 M for human U2OS and murine 3T3L1 cell lines, respectively
