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Cell viability was determined utilizing the CCK-8 assay

In triple-negative breast cancer (MDA-MB-231 and 4T1) cells, emodin targets nuclear receptor corepressor 2 (NCOR2) to upregulate seryl-tRNA synthetase (SerRS), suppressing VEGFA transcription and angiogenesis [440]

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When considering the aspect of compensating the abnormal melatonin secretion rather than the advancing effect of melatonin rhythm, it might be reasonable to opt for high doses of melatonin