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Description
Proteins were transferred onto a 0.2 m pore size nitrocellulose membrane (Novex-Life Technologies, Thermo Scientific) at 10 V for 2 h using 25 mM Tris, 190 mM glycine 20% methanol as transfer buffer
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Thigh The thigh muscle may also be used, depending on providers recommendation and patients preference
Future directions include precision modulation through epigenetic regulation and circRNAs, as well as personalized pharmacotherapy
