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Sampling Volume: lml at each time point is withdrawn and replaced with fresh medium

In a separate experiment, (MeHg) 2 S was also incubated with mouse liver supernatant centrifuged at 9000 g

These hallmarks of aging satisfy three conditions: (i) they become apparent with advancing age, (ii) experimentally perturbing them causes poor aging (iii) therapeutically intervening upon them prevents, delays or reverses aging phenotypes

A heat map of this data was generated using only the genera and metabolites that had previously demonstrated significant main effects of treatment group (Fig
