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The uptake of zinc in the liver in the acute phase reaction is linked to the regulation of gluconeogenesis, the control of reactive species, e.g., nitrogen monoxide, and the control of microbial growth through the nutritional immunity established by removing zinc and iron from blood while increasing copper
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The plasmid was then electroporated into plasmid-cured EcN and transformants were selected on LB agar supplemented with 12.5 g/mL chloramphenicol
