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The mechanism of action is closely linked to the multitargeted properties of natural products: Rikkunshito stimulates appetite by promoting ghrelin secretion and creates a more recoveryfriendly physiological environment by regulating gastrointestinal motility and hormone levels [60]

Source: SSRP Institute

For protein purification, harvested cells were resuspended with ice-cold lysis buffer (50 mM Tris-Cl pH 8.0, 300 mM NaCl, and cOmplete, EDTA-free protease inhibitor cocktail tablets (Roche)) at a ratio of 3.2 mL/g of cell pellet

Flow cytometry data were analyzed using CytExpert (v.2.3.0.84) (Beckman Coulter)
