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The final bead model was aligned to the AlphaFold2 full-length TDE0362 model using CIFSUP (Franke and Svergun, 2009) in RAW and fine-tuning of the fit in ChimeraX (Pettersen et al, 2021) (v1.6.1)

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Expression of the GRHPR gene is seen in most tissues with the highest levels in the liver and then the kidney

The obtained DNA fragments, upstream-aacC1 and tap1-TDE0362 were cloned into pGEM-T easy vector and fused by engineered cut sites XhoI as illustrated in Appendix Fig