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The heart tissue used in this project was prepared under the procedures carried out in project number 2019/33/N/NZ3/01649 as follows 13 : Isolation of rat hearts and perfusion with the Langendorff method Adult male Wistar rats weighing 200350 g were obtained from Mossakowski Medical Research Center, Polish Academy of Sciences, Warsaw, Poland

119 PEGylation extends the serum half-life, delays renal clearance, and reduces the immunogenicity of pegvisomant (B2036-PEG)

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