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Description
Western blotting The membrane fraction was prepared by disrupting cells with a French press, followed by ultracentrifugation (100,000 g , 1 h, 4 C) for purification

In mammals, two different CLCs have been defined, Cl channels (CLC-1/2/Ka/Kb) located at the plasma membrane and 2Cl /1H + antiporters (CLC-3/4/5/6/7) located at the internal membrane, which are involved in the regulation of muscle excitability, transepithelial Cl transport, and acidification of intracellular organelles, and mutations of CLCs lead to serious diseases 2
Eugene Arnold, MD, MEd, are with the Department of Psychiatry, Ohio State University Medical School, Columbus, OH

CPT: Pharmacometrics Syst Pharmacol
