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Signs of Vitamin B12 Deficiency Some common signs that you may be deficient in Vitamin B12 include fatigue or tiredness, weakness, difficulty concentrating or thinking clearly, pale skin, dizziness or light-headedness, loss of appetite, weight loss, constipation or diarrhea, numbness or tingling in the hands and feet, sore tongue, headaches and vision problems

Statistical analyses were carried out using GraphPad Prism software version 8.1.1

The resulting protein precipitates were separated from the medium by filtration and centrifugation methods (4000 g , 20 min, Centrifuge 5804R Eppendorf, Germany)

Compared to other nanoparticle formulations, this designed nanosystem offers advantages such as broad enzyme activity, fewer by-products, and simpler synthesis process