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Depending on the goals and specific needs for iPSC-HLCs as an in vitro model system, a dedifferentiated state might be preferable, for example, during investigation of liver regeneration mechanisms

After fixation and washing, sections were immersed in fresh -gal staining solution and incubated at 37 C overnight

This variability arises from differences in cellspecific requirements, the concentration and type of GFs needed, the use of serum versus serumfree formulations, and supplier pricing and production scales

Oxidative stress is defined as a redox imbalance where there is a decrease in natural antioxidants and excess formation of reactive oxygen species (ROS), which are chemically reactive molecules that contain oxygen