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Sigma) on ice for 20 min, and a nuclear run-on reaction was carried out at 30 C for 5 min in a reaction buffer [20 mM Tris pH 7.7, 200 mM KCl, 5 mM MgCl 2 , 1.25 mM dithiothreitol, 0.4 U/L SUPERase-In RNase Inhibitor (Fisher), 0.5% sarkosyl, 12.5 M biotin-11-CTP (Enzo Life Sciences), 12.5 M biotin-11-UTP (Biotium), 62.5 M ATP (Fisher), and 62.5 M GTP (Fisher)] to label newly transcribed RNA with biotin

In the blowfish Takifugu obscurus , the expression of 7 GST genes under Cd stress has been studied, and 5 of them (Mu, Omega, Zeta, Theta and MAPEG classes) are induced after 6 h exposure (Kim et al., 2010)

Eberhardt, M

The role of these fungi and bacteria in induction of resistance is discussed here