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Two separate rounds of filament picking on the entire dataset resulted in 2,374,969 and 2,865,445 particles, which were eventually merged into a pool of 492,224 particles after many rounds of 2D classification and removal of duplicate particles

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Interestingly, the observations extracted from computational analysis were in accordance with the calculated in vitro enzyme inhibition activities, i.e., stronger interaction of C3G (glycosylation of 3-OH group) via A-ring with binuclear copper ion results in its complete oxidation by the enzyme against EC and CH compounds

"Recombinant tumor suppressor TSC1 differentially interacts with Escherichia coli DnaK and human HSP70." ACS omega 5.30 (2020): 19131-19139.