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All cells were cultured in a DMEM medium (Gibco, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS), 100 U/mL of penicillin, and 100 g/ml of streptomycin in 5% CO 2 at 37C

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If personnel take pyridostigmine in advance of exposure, soman will be prevented from binding to a significant percentage of available synaptic AChE because the AChE binding sites will be transiently occupied by pyridostigmine, and so irreversible soman aging (formation of a covalent bond) to the enzyme cannot take place

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