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Although these examples can be criticized (for example, pinning out of the preparations could activate stretch-sensitive neurons), spontaneous activity in the ENS is readily observed by calcium imaging in primary cultures of enteric neurons (160) and spontaneous synaptic activity is detected in undissected preparations of the mouse colon (161), although the origins of this activity remain to be determined

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The peptide also helped rebalance immune hormones such as melatonin and testosterone, and improved the thymuss hormone production (9)

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