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Coimmunoprecipitation and Western blotting For coimmunoprecipitation assays, cells were washed twice with PBS and lysed with an NP-40 buffer (100 mM NaCl, 10 mM TrisHCl, pH 7.5, 0.2% NP-40, 10% glycerol) supplemented with cOmplete protease inhibitor cocktail (MilliporeSigma, Roche)
This aids the kidneys in removing extra fluids and minerals
Cellular signaling mechanisms
