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glutathione is synthesized from magnesium Synthase - an overview GLUTATHIONE SYNTHESIS - PMC
Description
Proteins were transferred onto a 0.2 m pore size nitrocellulose membrane (Novex-Life Technologies, Thermo Scientific) at 10 V for 2 h using 25 mM Tris, 190 mM glycine 20% methanol as transfer buffer

Nature 395 , 172177 (1998)
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