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All cell lines were confirmed to be free of mycoplasma, viral infections and contaminations with other cell lines, based on multiplex polymerase chain reaction (PCR) and single nucleaotide polymorphism (SNP) profiling
Bio-Rad) using the iBlot 2 dry blotting system (Invitrogen)
86 Moreover, other experimental approaches showed that substantial axon regeneration through lesions can be achieved by providing growth stimulating and chemoattractive factors, and this stimulated growth is attenuated (and not augmented) by disrupting astrocyte borders
J Nutr Biochem 2017
