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Description
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After centrifugation, cells were resuspended in the dark in PBS containing 50 g/mL propidium iodide (Sigma-Aldrich, USA) and 0.1 mg/mL RNaseA (20 g/mL PBS)
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Cycling MK-677 While MK-677 doesnt require PCT, its wise to cycle the compound to avoid desensitization and maintain long-term efficacy
