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Then, the cells were incubated at 37 C in a 5% CO 2 -containing environment

Other parameters included: inlet and flame ionization detector (FID) temperature at 290 C, MS transfer line temperature at 275 C, quadrupole temperature at 150 C, ion source temperature at 230 C, EI at 70 eV, m/z range of 33500

its simply present to balance the charge
