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Bacteriostatic water, due to its benzyl alcohol content, is not typically used for such applications, as the preservative could interact with certain drugs or patient conditions

On the day of the assay, plates were removed from the incubator and each well was washed twice with 100 l HBSS (Gibco, Life Technologies) and pre-incubated for 30 min at 37 C with 85 l HBSS per well

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