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Description
PMC 4303468
Transcriptomic analysis detected 2932, 4114, 3975, 2499, and 5905 DEGs after chilling stress in five materials, respectively
The impact of maternal age on aneuploidy in oocytes: Reproductive consequences, molecular mechanisms, and future directions
The pellet, after centrifugation, was resuspended in 3 mL lysis buffer 3 (10 mM Tris-Cl pH 8, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 0.1% Na-deoxycholate, 0.5% N-lauroylsarcosine, 1x Complete protease inhibitors (Roche)) and chromatin was sheared to an average size of 200600 bp by sonication for 20 cycles 30 s on/30 s off using Bioruptor Pico (Diagenode)
