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Results Screening for genes that promote copper toxicity in a cell model of Wilson disease To find new genes that might be targeted for reduction of Cu toxicity in Wilson disease we employed genome-wide shRNA screening in ATP7B-KO HepG2 cells, a bona fide WD cell model 12,13
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RNA design, folding, and primary modification were performed as described above
Additionally, ALA significantly increased the levels of anti-inflammatory mediators such as IL-10 and TGF- and improved macrophage M2b polarization
