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This review traces the historical trajectory from the early characterization of the biological effects of rapamycin to the elucidation of its molecular target and downstream pathways

Anaemic cats are lethargic, cold-intolerant, and have compromised oxygen delivery to tissues

However, it is worth keeping the solubility tag for some experiments such as GST-pulldowns which leverage the molecular interaction between GST and glutathione to investigate which proteins or other molecules that your protein of interest binds to (Figure 2)

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