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In this article: Nickel agarose beads Nickel affinity chromatography Nickel bead binding capacity and buffer compatibility What the color of the nickel beads tells me about my purification Comparing with other affinity chromatography techniques Impact of tag Cost of Reagents Elution Conditions Binding specificity References Nickel agarose beads Nickel is conjugated to agarose beads using one of two chelating agents: iminodiacetic acid (IDA) or nitrilotriacetic acid (NTA) (Figure 1)