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Upon reaching confluence, cultures were incubated with the adipogenesis induction cocktail (growth medium supplemented with 5 g ml 1 insulin, 1 M dexamethasone, 0.5 mM isobutylmethyxanthine and 1 M rosiglitazone) for day 12 of differentiation

Current theories concerning disease pathogenesis include an imbalance between protease and anti-protease activity, induced apoptosis of alveolar cells through deregulation of pathways involved in oxidative stress, chronic inflammation, and aberrant tissue remodeling that lead to the destruction of the extracellular matrix (ECM) in the lung [3, 4]

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