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Exogenous citrulline augments this loop because it is not a substrate for arginase, allowing more efficient restoration of the intracellular arginine pool

3) Sonicate the solution on ice using 180 one-second bursts at high intensity with a three-second cooling period until the bacterial liquid is clear

Items Needed] Distilled white vinegar (grocery store) Distilled water (grocery store) 0.2-0.22um sterile Whatman syringe filter 10mL syringe with a luer lock tip ~20-22 gauge needles (just the needles) Sterile glass vial (10-20mL) Alcohol prep pads sterile kind (70% isopropyl alcohol) Quick Guide]1

Nixon RA, Yang DS