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A Staphylococcus ASV (Stap_ASV5) was persistent in 27 of the 33 subjects in whom it was detected (0.82 persistence frequency)

[01:14:32] Chad: So check it out on the gram, you can see what they're all about

The design principle: Standard (often fails): His6 - TEV site - Target protein Better: His6 - TEV site - (GGS)2 - Target protein Even better: His6 - (GGS)2 - TEV site - (GGS)2 - Target protein Failure 2: Non-Specific Cleavage (The Protease Damages Your Protein) The problem: Proteases aren't perfectly specific

Non-reducing SDS-PAGE showed long smears for the oligomeric species of E1E2 and E2E1, indicating the presence of heterogeneous disulfide formation 31 , while E2E1-I53-50A showed one distinct band larger than 250 kDa, suggesting that E2E1-I53-50A forms a disulfide-bonded trimer (Fig