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Immediately after fixation the samples were washed once briefly with MilliQ and stained overnight at 37C with fresh SA--GAL solution (pH 6.0), containing 2,5 mM Na2HPO4, 7,4 mM Citric Acid, 0.15M NaCl, 2mM MgCl2, 5 mM Potassium Ferricyanide, 5 mM Potassium Ferrocyanide and 25ul/ml 4% X-gal in DMF

The following are some essential things to mention: Specific joint or bursa treated (e.g., tibiofemoral and/or patellofemoral joints of the right knee rather than just knee)

Practical and Administrative Reasons Resource Management: GP surgeries operate on tight schedules and budgets