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Description
J.RunswickM
The entire aorta and major organs (including the lungs, heart, liver, spleen, kidneys, and brain) were harvested for ex vivo imaging and fluorescence quantification using an IVIS Spectrum imaging system (PerkinElmer, USA) with an excitation wavelength of 645 nm and an emission wavelength of 665 nm
Lipids were extracted using the Folch procedure, and phosphorus was determined by a micromethod as described previously (7, 21)

Influence of Circadian Rhythm in the Eye: Significance of Melatonin in Glaucoma