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In contrast, -LA improved hyperinsulinemia and the higher levels of free fatty acids of the T2DM rats [129], increases antioxidant defense through Nrf2, and reduces inflammation in T2DM (decreases TNF- and Inhibit the expression of NLRP3 and downstream inflammatory factors IL-18 and IL-1) to prevent liver steatosis and relieve HFD-induced liver lipid accumulation in T2DM rats (Table 7)

2a, b, g, h)

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The forward and reverse primers were mixed with 100 ng cDNA and quantitative RT-PCR was performed using PowerUp SYBR Green Master Mix (Applied Biosystems, Darmstadt, Germany) or THUNDERBIRD SYBR qPCR Mix (TOYOBO, Japan)