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Degrees of skin erythema, induration, and scales were classified: 0, no symptoms

After centrifugation (1200 g, 10 min), the supernatant was discarded, and the resulting cell pellet was resuspended in culture medium supplemented with 5% of (FBS) (F4135, Sigma- Aldrich, Pozna, Poland) and seeded into cell culture flasks

Eventually, two ozone molecules are produced for every two OH radicals
