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The primers gene _pRS426_5UTR_fw and gene _pRS426_3UTR_rv contained a short homologous sequence to the MCS of the plasmid pRS426

Succinyl-CoA Synthetase, SCS (succinate-CoA ligase, succinate thiokinase): The conversion of succinyl-CoA to succinate, by succinyl-CoA synthetase (SCS), involves the use of the high-energy thioester of succinyl-CoA to drive synthesis of a high-energy nucleotide phosphate (GTP)

demonstrated well over 1 order of magnitude increase in signal relative to that of the instrument operated in its std

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