Direct radical scavenging by glutathione
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For spot assays, 10-fold serial dilutions were prepared starting at 10 6 cells, and 5 l of cell suspensions were spotted onto solid agar medium
GST activity was unchanged in vehicle-treated animals as compared to controls (not shown)
In contrast, WT cells transferred to gsh2 SM had less secreted melanin ( P = 0.077) and significantly less cell-wall bound melanin (Fig
