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Post-Immunoprecipitation Analysis After isolating the target DNA, various detection and quantification methods can be employed to study the isolated gene fragments

Among the predicted targets of miR-182 and miR-223, IGF-1R and FOXO3a were validated as their direct targets suggesting that HRT-mediated dysregulation of miR-182 and miR-223 induces the activation of IGF-1 signaling pathway by regulating IGF-1 signaling genes including IGF-1R and FOXO3a

All samples were lysed with 3 flash-freeze-thawing cycles using 80C and 37C, respectively

The 2016 world Health organization classification of tumors of the central nervous system: A summary