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They found that 6 of the 8 melanomas (75%), including both vulvar lesions, had multicomponent patterns
To determine the expected elution volume of PC-PLC, we performed a calibration of the HiLoad 16/600 Superdex 75 pg column using same buffer, and protein standards (IgG 150 kDa, transferrin 75 kDa, ovalbumin 43 kDa, scFvs 25 kDa, aprotinin 6.5 kDa)
Both PLD1 and PLD2, as well as several splice variants, hydrolyze phospholipids such that the polar head group, attached to the phosphate, is released with the other resulting product from this type of reaction being phosphatidic acid
