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The mitochondrial membrane potential in isolated mitochondria was assessed in quenching-mode, that is, when the level of Rho123 in the mitochondria is sufficiently high, its emission wavelength shifts towards the red, resulting in a reduction of the fluorescent signal at 527 nm 29

Processes such as glycolysis, glutamine uptake and catabolism, FAO, and polyamine synthesis and utilization are key energy pathways that drive cancer development, highlighting opportunities for metabolism-targeted cancer therapies

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