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The pcDNA5/FRT-MRP1 was digested with restriction enzymes x and y and ligated (blunt-end ligation) with the digested fragment A to obtain the vector expressing the chimera MRP1/MRP2
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In particular, ferritin levels above 2500 ng/mL are associated with an increased risk of morbidity and mortality and should be trigger intensification of chelation therapy (Ricchi et al
