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After SDS-PAGE separation and transfer to PVDF membranes, the membranes were blocked with 5% skim milk, followed by overnight incubation with primary antibodies at 4 C
Menopause and alterations in GH secretion
ELISA-based protein quantification indicated that LPS stimulated secretion of proinflammatory proteins including TNF-, Ccl2, and IL-6 from BV-2 cells (Fig
