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Description
Approximately 500 g of the mitochondrial pellet was resuspended in 200 L of nondenatured lysis buffer (WA-010, Invent Biotechnologies), a mild formulation designed to preserve native protein-protein interactions within the mitochondria while solubilizing mitochondrial membranes

A.GuptaJ.SinghA.et al

Repeat once or twice weekly as necessary
As an example, several enzymes in mitogen-activated protein kinase and phosphoinositide 3-kinase pathways, which are pivotal in mediating cellular responses to growth hormones and cytokines, are regulated directly by ROS [15,16,17]