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T., et al (2014)

There are five locations for in ovo delivery in chickens, namely, the air cell, embryo, yolk sac, allantoic membrane, and amniotic fluid ( in ovo technology was adapted over time for in ovo feeding of probiotics or prebiotics on embryonic day (e)18 or synbiotics delivery on e12 ( in ovo delivery on e12 is referred to as in ovo stimulation, whereas delivery on e1718 is known as in ovo feeding ( in ovo delivery for feeding purposes is late-term embryo and the amniotic fluid, respectively ( in ovo procedure on e12 involves delivery of bioactive compounds into the egg's air cell

Other mechanisms have also emerged, such as external auditory canal cooling, changing blood labyrinth barrier permeability, novel drug delivery, and the combination of multiple mechanism drugs

This assay is convenient and does not require special sample treatments, but its sensitivity can be influenced by factors such as solvent type and concentration, presence of hydrogen ions or metal ions, and the freshness of the DPPH reagent (Dawidowicz et al