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Under hyperglycemic conditions, dysfunction of the mitochondrial electron transport chain (ETC.) leads to excessive ROS production, subsequently inducing oxidative damage, mitochondrial DNA (mtDNA) mutations/leakage, and activation of inflammatory pathways such as the NLR family pyrin domain containing 3 (NLRP3) inflammasome and the cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING), thereby exacerbating local and systemic inflammation and hindering wound healing (Wang et al., 2025a

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Service Workflow and Quality Control for Targeted Tryptophan Pathway Analysis Analytical Platforms for Tryptophan Pathway Analysis Chromatography : Agilent 1290 Infinity II UHPLC System Reversed-phase C18 with sub-2 m particle columns for high peak capacity and separation of 20+ metabolites in a single run Mass Spectrometry : Agilent 6495C Triple Quadrupole LC/MS Mode: Multiple Reaction Monitoring (MRM)

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