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Samples were mixed with 2 denaturing buffer (60 mM Tris-HCl pH 6.8, 8 M urea, 4% (w/v) SDS, 20% (v/v) glycerol, and 0.01% (w/v) bromophenol blue) in a 1:1 volume ratio, resolved by SDS-PAGE on a 10% polyacrylamide gel, and stained by Coomassie brilliant blue R-250

Peak / Burst Discharge Rate This is the amperage the battery can sustain for a very short periodtypically 5 to 10 seconds

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Clinical Findings Signs and symptoms are mainly those caused by hypoproteinemia and in some instances by fat malabsorption: edema, ascites, poor weight gain, anemia, and specific vitamin (fat-soluble vitamins A, D, E, K) and mineral deficiencies