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Other nonspecific methods to inhibit GPxs include the use of L-buthionine sulfoximine to inhibit -glutamyl synthetase, an essential enzyme in GSH synthesis (298)

Mitochondrial dysfunction leads to the decrease of NAD + level, resulting in lysosomal acidification and impaired autophagy, and induces ferroptosis, involving the accumulation of iron in lysosomes and lipid peroxidation

The formation of isothiocyanates from glucosinolates is also influenced by several other factors (3), including pH value, ferrous ions, and specifier proteins such as epithiospecifier protein (ESP), nitrile-specifier protein (NSP), and thiocyanate-forming protein (TFP)

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