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J Biol Chem (2009) 284(30):1993744.10.1074/jbc.M109.014308 258 LiXBartkeABerrymanDEFunkKKopchickJJListEOet alDirect and indirect effects of growth hormone receptor ablation on liver expression of xenobiotic metabolizing genes

The cells were washed with PBS twice before staining with X-gal solution [1 mg/mL 5-bromo-4-chloro-3inolyl--D-galactoside (Boehringer Manneheim #745-740) in N,N-dimethylformamide, 5 mM potassium ferricyanide crystalline (#P-8131), 5 mM potassium ferricyanide trihydrate (#P-3289), 2 mM magnesium chloride, and 1x PBS, pH 6.0

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