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Differential expression analysis from scRNA-seq To find genes differentially expressed between cell clusters or pre-defined populations, a custom pipeline combining edgeR 83 and Seurats FindMarkers tool was used
[DOI] [PubMed] [Google Scholar] 153.Hidalgo C, Aracena P, Sanchez G, Donoso P

Cell debris was removed by centrifugation at 4,400 g at 4 C for 30 min

6.3 Lipid metabolic reprogramming Normal lipid metabolism mainly consists of three core pathways: fatty acid -oxidation (FAO, a catabolic process), fatty acid synthesis, and cholesterol synthesis (95)