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The time-resolved data obtained by single-cell confocal microscopy of green fluorescent protein (GFP) reporters and qPCR of the Nrf2 pathway components complemented with siRNA knock down experiments, is accurately described by the calibrated mathematical model

Ferroptosis is characterized by a substantial anticipation of lipid peroxidation

Phospholipids and their hydroxy and hydroperoxy derivatives, including 15-HETE-PE and 15-HpETE-PE, were analyzed by LC/MS using a Dionex Ultimate 3000 HPLC system coupled on-line to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) using a normal phase column (Luna 3 m silica C18(2) 100 , 150 2.0 mm, Phenomenex)