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Next, 50 L of a 4 mM NADP + (Neta Scientific, SPCM-N1131-OM) and 20 U/mL IDH1 (Sigma-Aldrich, I1877) solution, and 50 L of 10 mM sodium citrate were sequentially added to initiate the assay
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Western blotting was used to analyze the changes in VEGF in different treatment groups
